Vagus Activity Versus Sympathetic: Electroacupuncture before Stress at PC6 or ST36 alleviates myocardial inflammation: Impli
This is an article to bookmark for vagus activity versus sympathetic activity in acupuncture. It looks at heart attacks produced in lab mice after a preconditioning with acupuncture at two often used acupuncture points, PC ^ and St 36. We’ve discussed these points on the blog before. Especially ST36 in regards to vagal activity and stimulation settings. Interestingly, PC 6 was more implicated in the sympathetic nervous system, and St36 more with vagal activity. This makes some sense since St 36 has been shown to be close to nerves with specific receptors for vagal activity. PC 6 involvement in sympathetic activity is fascinating .
The obvious limiting aspect of this study is making it useful in the clinic. You would have to be able to predict when an MI was about to come on with a few days lead time.
But it does NOT make is irrelevant since the pathways invoked tell us quite a bit, and may be useful in other ways.
Chen L, Yang W, Shi J, Jiang M, Peng R, Fu S, Zhang T, Cao X, Yu M, Lu S. Electroacupuncture preconditioning at PC6 or ST36 alleviates myocardial inflammation and promotes M2 macrophage phenotypic polarization via distinct autonomic pathways. Acupunct Med. 2026 Aug 14:9645284261464194. doi: 10.1177/09645284261464194. Epub ahead of print. PMID: 42599045.
Abstract Vagus and Sympathetic Involvment
Background: Acupuncture at different traditional acupuncture point locations activates different autonomic pathways and modulates systemic inflammation. Electroacupuncture preconditioning (EAP) at PC6 or ST36 has been demonstrated to be cardioprotective during acute myocardial ischemia (MI) injury, but the mechanism remains unclear. As inflammation is crucial for repair post-MI, we aimed to investigate whether the protective effect of EAP at PC6 or ST36 is related to the suppression of inflammation and whether autonomic pathways are involved.
Methods: Mice underwent ligation of the left anterior descending (LAD) coronary artery to generate model MI after 3 days of EAP at PC6 or ST36. Echocardiography and triphenyltetrazolium chloride (TTC) staining were used to evaluate cardiac function and myocardial infarction area. Cardiac troponin (cTn)I and norepinephrine (NE) were measured by enzyme-linked immunosorbent assay (ELISA). Flow cytometry was employed to determine macrophage M1/M2 phenotypic polarization, and the levels of pro- and anti-inflammatory cytokines in cardiac tissue were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). In addition, unilateral cervical vagotomy and chemical sympathetic blockade were employed to clarify specific autonomic pathways.
Results: EAP at PC6 or ST36 preserved cardiac function, reduced myocardial infarct size, decreased serum cTnI levels, promoted M2 macrophage polarization and decreased the cardiac pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-18 and nucleotide-binding oligomerization domain (NOD)-like receptor protein 3 (NLRP3). Compared to EAP at ST36, EAP at PC6 yielded a better cardioprotective effect and regulated the anti-inflammatory cytokine transforming growth factor (TGF)-β. Moreover, sympathetic nerves were involved in the protective effect of EAP at PC6, while the vagus nerve was necessary for EAP at ST36.
Conclusion: Our findings suggest that EAP at PC6 or ST36 activates different autonomic pathways and modulates M2 macrophage polarization, resulting in the suppression of myocardial inflammation.
Keywords: autonomic nervous system; electroacupuncture preconditioning; inflammation; macrophage polarization; myocardial ischemic injury.